abstract：:In order to analyze the epitope structure of carcinoembryonic antigen (CEA) and the idiotype network system, seven anti-idiotypic monoclonal antibodies (anti-Id MoAbs) were generated from a BALB/c mouse immunized with anti-CEA MoAb P1-356, which recognized a synthetic peptide P1 of CEA, domain I. These MoAbs were divi...

journal_title：Journal of clinical laboratory analysis

authors： Tsujisaki M,Masuya J,Okada Y,Jinnohara T,Sasaki S,Yachi A,Imai K

更新日期：2002-01-01 00:00:00

abstract：:Second- and higher-order degradation reactions sometimes cannot be approximated with linear or exponential relationships and need to be appropriately modeled. Events above the COULTER HmX Analyzer white blood cell (WBC) counting threshold were recorded for the HmX PAK reagent system stored at five elevated temperature...

journal_title：Journal of clinical laboratory analysis

authors： Magari RT,Murphy KP,Fernandez T

更新日期：2002-01-01 00:00:00

abstract：:We here describe an ion-exchange high-performance liquid chromatography technique with electrochemical detection for rapid quantification of glutathione, homocysteine, cysteinylglycine, and methionine. The analytical validation of the technique showed within-assay and between-assay coefficients of variation between 3....

journal_title：Journal of clinical laboratory analysis

authors： Houze P,Gamra S,Madelaine I,Bousquet B,Gourmel B

更新日期：2001-01-01 00:00:00

abstract：:The detection of hepatitis C virus (HCV) RNA by nucleic acid amplification techniques is the method of choice to differentiate between ongoing and past infection, and can be used to monitor the course of HCV infection. In this study, we evaluated the performance characteristics of a newly developed transcription-media...

journal_title：Journal of clinical laboratory analysis

authors： Ross RS,Viazov SO,Hoffmann S,Roggendorf M

更新日期：2001-01-01 00:00:00

abstract：:Gross cystic breast disease (GCBD) is the most common benign disease of the human female breast, and patients with GCBD have an increased risk of breast cancer. The aim of this study was to evaluate the distribution inside apocrine cells and in breast cyst fluids aspirated from gross cysts of prostate-specific antigen...

journal_title：Journal of clinical laboratory analysis

authors： Malatesta M,Mannello F,Sebastiani M,Gazzanelli G

更新日期：2001-01-01 00:00:00

abstract：:p21, an important regulator of the cell cycle, acts as a mediator of the growth-suppressing and -promoting functions of p53. We aimed to investigate the association between codon 31 polymorphisms of p21 gene and endometriosis. Women were divided into two groups: endometriosis (n = 102) and nonendometriosis (n = 119). ...

journal_title：Journal of clinical laboratory analysis

authors： Hsieh YY,Tsai FJ,Chang CC,Chen WC,Tsai CH,Tsai HD,Lin CC

更新日期：2001-01-01 00:00:00

abstract：:Adenosine deaminase (ADA) exists as two isoenzymes, ADA(1) and ADA(2). It appears that the ADA(2) isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA(2). The aim of this prospective study was to analyse ADA isoenzymes in the CSF of patie...

journal_title：Journal of clinical laboratory analysis

authors： Schutte CM,Ungerer JP,du Plessis H,van der Meyden CH

更新日期：2001-01-01 00:00:00

abstract：:The relationship between the levels of serum cystatin C and the prognostic stages of IgA nephropathy was determined in a multicenter trial in Japan. The levels of serum cystatin C in patients with IgA nephropathy were measured using the Dade Behring N Latex Cystain C assay. In 1995, the Joint Committee of the Special ...

journal_title：Journal of clinical laboratory analysis

authors： Tomino Y,Suzuki S,Gohda T,Kobayashi M,Horikoshi S,Imai H,Saito T,Kawamura T,Yorioka N,Harada T,Yasumoto Y,Kida H,Kobayashi Y,Endoh M,Sato H,Saito K

更新日期：2001-01-01 00:00:00

abstract：:The levels of serum IgA and C3 in patients with IgA nephropathy were determined using international standard serum (IFCC/CRM470) in a multicenter trial in Japan. The ratio of serum IgA to C3 (serum IgA/C3 ratio) without any information from renal biopsy was used for the diagnosis of IgA nephropathy. Three hundred and ...

journal_title：Journal of clinical laboratory analysis

authors： Tomino Y,Suzuki S,Imai H,Saito T,Kawamura T,Yorioka N,Harada T,Yasumoto Y,Kida H,Kobayashi Y,Endoh M,Sato H,Saito K

更新日期：2000-01-01 00:00:00

abstract：:Recombinant HIV-1 p66 (rp66, a subunit of reverse transcriptase (RT), a heterodimer of p66 and p51) was produced in Escherichia coli in three different ways. First, rp66 was produced as a part of the fusion protein of lacZ protein and HIV-1 pol protein consisting of three components: protease (p10), RT (p51/p66), and ...

journal_title：Journal of clinical laboratory analysis

authors： Hashinaka K,Nishikata I,Hashida S,Adachi A,Oka S,Ishikawa E

更新日期：2000-01-01 00:00:00

abstract：:Early monitoring of HCVRNA during interferon treatment may allow clinicians to obtain important information that could help them to adopt therapeutic decisions in individual cases. The hepatitis C virus infection is highly dynamic and a daily high dose of IFN may induce a decline of viremia of 95+/-10% of baseline val...

journal_title：Journal of clinical laboratory analysis

authors： Magalini A,Puoti M,Putzolu V,Quiros-Roldan E,Forleo MA,Rossi S,Zaltron S,Spinetti A,Zanini B,Zonaro A,Solfrini R,Carosi G

更新日期：2000-01-01 00:00:00

abstract：:C-peptide is secreted in equimolar concentrations with insulin, and is often measured to assess pancreatic beta-cell function. C-peptide analysis is most often performed by radioimmunoassay (RIA) which has several disadvantages. We evaluated an automated, chemiluminescent immunoassay for C-peptide in terms of precisio...

journal_title：Journal of clinical laboratory analysis

authors： Hardy RW,Cohn M,Konrad RJ

更新日期：2000-01-01 00:00:00

abstract：:The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a consi...

journal_title：Journal of clinical laboratory analysis

authors： Noguera NI,Tallano CE,Bragós IM,Milani AC

更新日期：2000-01-01 00:00:00

abstract：:The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modifi...

journal_title：Journal of clinical laboratory analysis

authors： Gutiérrez J,Rodríguez MJ,De Ory F,Piédrola G,Maroto MC

更新日期：1999-01-01 00:00:00

abstract：:The authors compare efficacy of two ELISA assays (one supplied by DIAMEDIX [Delta Biological s.r.l.], and the other by RADIM [RADIM I]) in detecting total anti-H. pylori antibodies, and of two further ELISA methods (one supplied by EUROSPITAL [Helori CTX IgG] and the other by RADIM [RADIM 2]) in identifying anti-CagA ...

journal_title：Journal of clinical laboratory analysis

authors： Basso D,Stefani A,Brigato L,Navaglia F,Greco E,Zambon CF,Piva MG,Toma A,Di Mario F,Plebani M

更新日期：1999-01-01 00:00:00

abstract：:Elevated serum chromogranin A (CgA) levels have been detected in patients with prostate cancer who have developed resistance to hormonal therapy. We would like to reexamine these cases by using serial specimens to determine whether such elevated levels are also detectable in prostate cancer patients not undergoing hor...

journal_title：Journal of clinical laboratory analysis

authors： Wu JT,Wu TL,Chang CP,Tsao KC,Sun CF

更新日期：1999-01-01 00:00:00

abstract：:We described an automated enzymatic assay for conjugated bilirubin (Bc) in serum using the Iatro D-Bil kit, with bilirubin oxidase (EC 1.3.3.5 BOD) from Myrothecium species. The specificity of the enzyme in the Iatro D-Bil kit was examined by analyzing unconjugated bilirubin (Bu), delta bilirubin (Bdelta), and Bc with...

journal_title：Journal of clinical laboratory analysis

authors： Kimura S,Iyama S,Yamaguchi Y,Hayashi S,Yanagihara T

更新日期：1999-01-01 00:00:00

abstract：:The elevation of chylomicrons and chylomicron remnants in plasma would lead to hyperlipidemia and other complications. Apo B-48, which is translated and produced in the adult intestine from the same gene as Apo B-100, is considered to be an essential component of chylomicrons and chylomicron remnants. Using a peptide ...

journal_title：Journal of clinical laboratory analysis

authors： Uchida Y,Kurano Y,Ito S

更新日期：1998-01-01 00:00:00

abstract：:Antibodies to neutrophil cytoplasmic antigens (ANCA) targeted toward granule enzymes have been recognized as a valuable diagnostic tool in the detection of Wegener's granulomatosis and systemic vasculitides. However, the most commonly used method of detection, the indirect immunofluorescence assay, is prone to false-p...

journal_title：Journal of clinical laboratory analysis

authors： Streicher J,Fabian B,Herkner K,Pointner H,Bayer PM

更新日期：1998-01-01 00:00:00

abstract：:Diurnal variations in the excretion of bone resorption markers were assessed in order to identify the type of urine collection which provides the most information on bone resorption rate and its relation to measuring bone dynamics in a postmenopausal population. Sixty women, ages 43-67 and without disease or treatment...

journal_title：Journal of clinical laboratory analysis

authors： Zaninotto M,Bernardi D,Ujka F,Bonato P,Plebani M

更新日期：1998-01-01 00:00:00

abstract：:Type IV collagen is a major component released from the glomerular and tubular basement membranes. To investigate the alteration of renal type IV collagen turnover in early stage diabetic nephropathy, urinary type IV collagen was measured by a highly sensitive one-step sandwich enzyme immunoassay (EIA). Urinary sample...

journal_title：Journal of clinical laboratory analysis

authors： Iijima T,Suzuki S,Sekizuka K,Hishiki T,Yagame M,Jinde K,Saotome N,Suzuki D,Sakai H,Tomino Y

更新日期：1998-01-01 00:00:00

abstract：:The immune complex transfer enzyme immunoassay for antibody IgG to HIV-1 gp41 antigen was developed using two synthetic peptides. An aliquot (10 microl) of serum samples from HIV-1 seropositive subjects was incubated simultaneously with 2,4-dinitrophenyl-bovine serum albumin-synthetic HIV-1 gp41 peptide conjugates and...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Ishikawa S,Hashinaka K,Nishikata I,Oka S,Ishikawa E

更新日期：1998-01-01 00:00:00

abstract：:By using commercially available ELISA kits, serum IL-6 and TNF-alpha levels in healthy adults, and the levels of various cytokines in patients with primary biliary cirrhosis or chronic viral liver diseases, were investigated. IL-6 levels of healthy subjects were distributed in a wide range, and the distribution patter...

journal_title：Journal of clinical laboratory analysis

authors： Yamashiki M,Kosaka Y,Nishimura A,Watanabe S,Nomoto M,Ichida F

更新日期：1998-01-01 00:00:00

abstract：:We studied the reliability of new indirect tests in the diagnosis of acute infection by Epstein-Barr virus (EBV). Studied for all samples were method 1, the heterophil antibodies (Abs) (Monolatex, Biokit, Germany); method 2, the IgM Abs to EBV with ELISA tests (antigen pools, Enzygnost, Behring-werke, Germany); method...

journal_title：Journal of clinical laboratory analysis

authors： Gutiérrez J,Rodríguez M,Maroto C,Piédrola G

更新日期：1997-01-01 00:00:00

abstract：:Congenital hypoplastic anemias are a rare and heterogeneous group of disorders. This paper reviews new molecular diagnostic tests in two distinct forms of congenital anemias, anemia due to transplacental infection with B19 parvovirus and Fanconi anemia. In both instances, molecular assays making use of amplification o...

journal_title：Journal of clinical laboratory analysis

authors： Auerbach AD,Verlander PC,Brown KE,Liu JM

更新日期：1997-01-01 00:00:00

abstract：:We developed enzyme immunoassays for human anti-Pichia pastoris components (PPC) IgG and anti-PPC IgE antibody titers. Anti-PPC IgG antibody assay were performed using antigen-coated plate and anti-human IgG peroxidase conjugate. The intra- and interassay coefficients of variation (CV) of anti-PPC IgG antibody were 1....

journal_title：Journal of clinical laboratory analysis

authors： Ohtani W,Kobayashi K,Ohmura T

更新日期：1997-01-01 00:00:00

abstract：:A microparticle-enhanced nephelometric immunoassay was developed for lactoferrin quantitation in human milk. It is based on the nephelometric measurement of the light scattered during the competitive immuno-agglutination of a microparticle-lactoferrin conjugate with an antilactoferrin antiserum. This immunoassay is se...

journal_title：Journal of clinical laboratory analysis

authors： Cuillière ML,Montagne P,Molé C,Béné MC,Faure G

更新日期：1997-01-01 00:00:00

abstract：:An optimized reaction condition for amplification of influenza A virus RNA, by thermus thermophilus (Tth) DNA polymerase-based PCR, incorporating uracil N glycosylase (UNG) and dUTP in the reaction has been determined. dUTP could not be substituted for all dTTP sites when UNG was present in the reaction. The relative ...

journal_title：Journal of clinical laboratory analysis

authors： Poddar SK,Sawyer MH,Connor JD

更新日期：1997-01-01 00:00:00

abstract：:Numerous detection methods for Helicobacter Pylori (H. pylori) have been developed with varying degrees of purported diagnostic utility. We have developed a rapid nonradioactive in situ hybridization (ISH) method for H. pylori detection in paraffin-embedded tissue and assessed its relative diagnostic performance by re...

journal_title：Journal of clinical laboratory analysis

authors： Barrett DM,Faigel DO,Metz DC,Montone K,Furth EE

更新日期：1997-01-01 00:00:00

abstract：:The experimental and clinical effectiveness of nasal salmon calcitonin (SCT) for treatment of osteoporosis in humans has been well established, but none is known yet about the pharmacokinetic property in relation to therapeutic efficacy, especially when used in a therapeutic dose range. This preclinical study was desi...

journal_title：Journal of clinical laboratory analysis

authors： Nakamuta H,Kohno T,Ichikawa M,Hoshino T,Watabe K,Koida M

更新日期：1997-01-01 00:00:00

abstract：:Rats exposed to constant light develop polycystic ovarian (PCO) disease with persistent estrus, representing an estrogen-dominant condition. Herein, we report that fluctuations seen in the vaginal microflora in cyclic rats were not observed in PCO rats with persistent estrus. The vaginal-cervical mucosa of PCO rats sh...

journal_title：Journal of clinical laboratory analysis

authors： Singh KB,Mahajan DK,Tewari RP

更新日期：1996-01-01 00:00:00

abstract：:Previously, an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for p24 antigen of HIV-1 was developed. The immune complex comprising 2,4-dinitrophenyl-biotinyl-bovine serum albumin-rabbit anti-p24 Fab' conjugate, p24 antigen, and rabbit anti-p24 Fab' -beta-D-galactosidase conjugate was t...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Hashinaka K,Nishikata I,Saito A,Takamizawa A,Shinagawa H,Ishikawa E

更新日期：1996-01-01 00:00:00

abstract：:The technique that allows for in situ detection of PCR-amplified DNA and cDNA has improved remarkably quickly over the past 5 years. As of this writing, one can expect good results after a relatively quick "learning curve." The technique demands some skill in working with glass slides and cells/tissues. Thus, I recomm...

journal_title：Journal of clinical laboratory analysis

authors： Nuovo GJ

更新日期：1996-01-01 00:00:00

abstract：:In order to establish a rapid staining method sensitive enough to stain the protein bands on cellulose acetate membrane and suitable for the analysis of urinary proteins in healthy subjects, we conducted a detailed examination of Kovarik's method designed for the analysis of proteins in cerebrospinal fluid in detail. ...

journal_title：Journal of clinical laboratory analysis

authors： Hiratsuka N,Shiba K,Shinomura K,Hosaki S

更新日期：1996-01-01 00:00:00

abstract：:The importance of the neutrophil function to host defense and tissue damage has been widely recognized. However, the usefulness of the correlation analysis between phagocytic activity and superoxide production of neutrophils is unknown. We investigated the relationship by histochemical NBT assay using rats and mice ex...

journal_title：Journal of clinical laboratory analysis

authors： Kuriyama T,Machida K,Suzuki K

更新日期：1996-01-01 00:00:00

abstract：:The present study was designed to evaluate whether the use of acid citrate dextrose (ACD) Formula A may enhance the survival of platelets during storage, thus allowing the continuance of platelet studies over the period of 2-3 hours usually recommended. For this purpose the effects of time on in vitro platelet respons...

journal_title：Journal of clinical laboratory analysis

authors： Pignatelli P,Pulcinelli FM,Ciatti F,Pesciotti M,Ferroni P,Gazzaniga PP

更新日期：1996-01-01 00:00:00

abstract：:The shortcomings of current methods of basophil enumeration detract from the clinical value of the basophil count. Moreover, sophisticated and costly techniques of automated basophil counting hardly can be validated for lack of a suitable reference method. We investigated whether a flow cytometric technique using doub...

journal_title：Journal of clinical laboratory analysis

authors： Hübl W,Andert S,Erath A,Streicher J,Bayer PM

更新日期：1996-01-01 00:00:00

abstract：:Medical treatment for Helicobacter pylori (Hp) infection is now recommended in several types of gastroduodenal disease, and its success is usually monitored by hystology. The end-points of our work were to identify the most suitable serum index of Hp eradication among pepsinogen A (PGA), pepsinogen C (PGC), PGA/PGC ra...

journal_title：Journal of clinical laboratory analysis

authors： Plebani M,Basso D,Scrigner M,Toma A,Di Mario F,Dal Bò N,Samloff IM

更新日期：1996-01-01 00:00:00

abstract：:A microparticle-enhanced nephelometric immunoassay was developed for plasminogen quantitation in human plasma. It is based on the nephelometric measurement of the light scattered by microparticle clusters formed during a sandwich reaction between plasminogen, microparticle--anti-plasminogen conjugate, and the free ant...

journal_title：Journal of clinical laboratory analysis

authors： Marchal E,Montagne P,Cuillière ML,Béné MC,Faure G

更新日期：1996-01-01 00:00:00

abstract：:To investigate the influence of race, cigarette smoking, and immunologic parameters on serum immunoglobulins, we analyzed serum IgG, IgA, and IgM levels in 455 healthy adults. The study population ranged in age from 20 to 69 years, including 282 whites and 173 blacks, 181 never-smokers, 93 ex-smokers, and 181 current ...

journal_title：Journal of clinical laboratory analysis

authors： Tollerud DJ,Brown LM,Blattner WA,Weiss ST,Maloney EM,Kurman CC,Nelson DL,Hoover RN

更新日期：1995-01-01 00:00:00